Real time PCR: Easy PGX line

EasyPGX Ready is the best-in-class RT-PCR diagnostic solution for oncohematology with a fast and simple workflow based on dry and ready-to-use reagent format.

One-step real-time PCR
Validation from RNA extracted from marrow or peripheral blood and leukocyte suspensions (buffy coat)
TAT from RNA to result < 80 minutes
Hands-on time <10 minutes
The same thermal profile shared between all RNA applications.

Kits include:

5 quantification standards (105 -101 copies/rxn) calibrated with IRMM ERM-AD623 BCR-ABL1.
p210 RNA reference standard for results allignments to international scale (%IS)

Maximum sensitivity:

International scale (%IS) BCR-ABL p210: 0.0015%
BCR-ABL p190: 0.001%

Flexibility

Same thermal profile for all EasyPGX qualitative and quantitative RNA kits
Up to 41 clinical samples for each single run

Automatic data analysis:

Dedicated software solution with automatic calculation of molecular response (MR) and international scale (%IS).

*Use extraction solutions compatible with those described in the user manual.

EasyPGX Real-Time PCR System

RT-PCR instrument with 96 wells peltier block
Up to 6 LED optical channels
Integrated touch screen
Automatic data analysis and interpretation software
System complete of all accessory instrumentation needed for EasyPGX ready workflow
CE-IVD

EasyPGX Analysis Software: automatic data analysis solution Control Mix and Mutation Assay Analysis Report EasyPGX Analysis Software is the automatic data analysis software dedicated to the use with EasyPGX ready kits.

CE- IVD
Complete automated data analysis with a turnaround time < 5 min
No cloud or external data analysis solution
Totally automatic data analysis interpretation with the possibility to check raw data in one single software
Run and sample automatic QC
Automatic calculation of molecular response (MR) and International Scale (%IS)
LIMS connectivity
Data export and report on different common formats

EasyPGX ready BCR-ABL p210 code RT039 (48 test - CE-IVD)

Kit for quantification — by One Step Real-Time RT-PCR — of BCR-ABL fusion variants b2a2 (e13a2) and b3a2 (e14a2), encoding a 210 kDa chimeric protein, in total RNA isolated from peripheral blood or bone marrow.

EasyPGX ready BCR-ABL p190 code RT040 (48 test - CE-IVD)

Kit for quantification — by One Step Real-Time RTPCR — of BCR-ABL fusion variant e1a2 encoding a 190 kDa chimeric protein, in total RNA isolated from peripheral blood or bone marrow.

EasyPGX ready WT1 Quant code RT046 (48 test - CE-IVD)

Kit for quantification — by One Step Real-Time PCR — of Wilms tumour 1 (WT1) gene transcript, in total RNA isolated from peripheral blood or bone marrow and leukocyte suspensions (derived from buffy coat).