PRINCIPLE OF THE METHOD:
The “Easy® SARS-CoV-2 WE” kit contains 2 oligo mixes:
Oligo-mix 1: allows the analysis of two SARS-COV-2 specific genes, N and RdRp, and one endogenous control gene by One-step Real-Time RT-PCR. Detection is performed using FAM- and ROX-labelled fluorescent probes for detection of viral targets and a HEX-labelled probe for detection of an endogenous control gene. Amplification of the endogenous control gene allows verification of the correct sample preparation procedure and the possible presence of inhibitors, which may cause false negative results.The target regions used are recommended by WHO and CDC.
Oligo-mix 2: allows the analysis of two mutations in the S gene (HV69-70 del and N501Y) and the E gene specific for the subgenus Sarbecovirus. Detection is by means of fluorescent probes labelled with FAM and ROX for mutation detection and a HEX-labelled probe for the E gene, which is used as an additional confirmation of the presence of the virus (3 genes) and as an internal reaction control. that allows the simultaneous detection of the three viral targets and the endogenous control gene. The target regions used are recommended by WHO and CDC.