The kit allows the manual extraction of circulating free DNA (cfDNA) from plasma. The kit Helix® Circulating Nucleic Acid, in association with the kit Easy® EGFR, enables the mutational analysis of EGFR gene in the circulating tumor DNA (liquid biopsy) when the tumor tissue is not evaluable, according to the EMA/129677/2014 recommendations of September 25th 2014.