Stefano Reale et al., PrP Gene Polymorphism in Medieval Remains of Sicilian Sheep. Open Journal of Animal Sciences

Stefano Reale1, Mario Cosenza1, Luca Sìneo2, Letizia Arcoleo2, Gabriele Zaffora1, Daniele Macrì1, Fabrizio Vitale1

1Molecular Biology Department, Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Palermo, Italy
2Laboratory of Anthropology, STEBICEF Department, University of Palermo, Palermo, Italy


Encephalopathy in sheep was at first described in Ireland in 1732 and was called scrapie. Ancient DNA in archaeogenetics represents an effective method to evaluate the ancestral pedigree of living animals and track evolutionary changes occurred between the past and the present day. Since several point mutations are today widely described in modern scrapie, no data about both sequence and frequency are still available for the prion protein (PrP) gene in ancient breeds. In order to evaluate whether the haplotypes distribution in ancient sheep differed from those of the modern population we evaluated polymorphism at four well know codons of the Prp Open Reading Frame. In the present work, we collected 37 medieval sheep bone remains found at the Calathamet (n = 11), Palazzo Bonagia (n = 12) and Palazzo Steri (n = 14) Sicilians archeological sites and dated back between 9th – 15th century. Single nucleotide polymorphisms (SNPs) at codon