Elisabetta Falvo et al., SNPs in DNA repair or oxidative stress genes and late subcutaneous fibrosis in patients following single shot partial breast irradiation. Journal of Experimental & Clinical Cancer Research

Elisabetta Falvo¹, Lidia Strigari², Gennaro Citro¹, Carolina Giordano³, Genoveva Boboc³, Fabiana Fabretti³, Vicente Bruzzaniti², Luca Bellesi², Paola Muti^4,5, Giovanni Blandino⁶ and Paola Pinnarò³

¹Laboratory of Pharmacokinetic/Pharmacogenomic, Regina Elena National Cancer Institute
²Laboratory of Medical Physics and Expert Systems, Regina Elena National Cancer Institute
³Department of Radiation Oncology, Regina Elena National Cancer Institute
⁴Department of Oncology Juravinski Cancer Center, McMaster University Hamilton
⁵Department of Public Health, Harvard University
⁶Translational Oncogenomic, Regina Elena National Cancer Institute

Abstract

Background
The aim of this study was to evaluate the potential association between single nucleotide polymorphisms related response to radiotherapy injury, such as genes related to DNA repair or enzymes involved in anti-oxidative activities. The paper aims to identify marker genes able to predict an increased risk of late toxicity studying our group of patients who underwent a Single Shot 3D-CRT PBI (SSPBI) after BCS (breast conserving surgery).

Methods
A total of 57 breast cancer patients who underwent SSPBI were genotyped for SNPs (single nucleotide polymorphisms) in XRCC1, XRCC3, GST and RAD51 by Pyrosequencing technology. Univariate analysis (ORs and 95% CI) was performed to correlate SNPs with the risk of developing ? G2 fibrosis or fat necrosis.

Results
A higher significant risk of developing ? G2 fibrosis or fat necrosis in patients with: polymorphic variant GSTP1 (Ile105Val) (OR = 2.9; 95%CI, 0.88-10.14, p = 0.047).

Conclusions
The presence of some SNPs involved in DNA repair or response to oxidative stress seem to be able to predict late toxicity.

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