Easy PGX Line

Easy PGX Line 2017-09-08T12:44:08+00:00

EasyPGX® product line – key features

Ready to use: reagents are delivered in 8-well strips preloaded with a complete master mix in a dry, room temperature and stable format.

Easy to use: no need for freezing, thawing or pipetting on ice and the few remaining pipetting steps minimize the risk of errors or contaminations.

High sensitivity: limit of detection as low as 0.5%.

Flexible sample requirement: low DNA input from a variety of sources, including FFPE and plasma.

Turnaround time: from tissue to result in less than 3 hours with only 10 minutes of hands-on time.

Quality assurance: manufactured under ISO 13485.

Regulatory: kits have been designed, developed and validated in accordance with the Directive 98/79/EC on in vitro diagnostic medical devices.

EasyPGX system workflow: from tissue to result in less than 3 hours.

Main features Detection of the main mutations of exon 2 (codons 12, 13), of exon 3 (codons 59, 61) and of exon 4 (codons 117, 146) of the gene KRAS using 8
oligo mixes. Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene. A specific oligo control mix enables
the evaluation of the quality and the quantity of the DNA in each sample.

Controls  The kit is provided with the following controls:

  • Positive control sample containing a mixture of synthetic DNA sequences that correspond to each mutation detected by this kit in a background of wild-type genomic DNA.
  • Negative control.

Sensitivity The kit allows the detection of low percentages of mutated allele in presence of high amounts of wild-type genomic DNA by real-time amplification with sequence-specific probes marked with FAMb and HEX. LOD down to 0.5%.

Starting material The kit allows the analysis of DNA extracted from fresh, frozen, formalin-fixed parain-embedded (FFPE) tissues and plasma*.

*Please note that extraction from plasma is sold separately (cat.no. H8040)

Main features Detection of the main mutations of codon 600 of the gene BRAF using 4 oligo mixes. Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene. A specific oligo control mix enables the evaluation of the quality and the quantity of the DNA in each sample.

Controls  The kit is provided with the following controls:

  • Positive control sample containing a mixture of synthetic DNA sequences that correspond to each mutation detected by this kit in a background of wild-type genomic DNA.
  • Negative control.

Sensitivity The kit allows the detection of low percentages of mutated allele in presence of high amounts of wild-type genomic DNA by real-time amplification with sequence-specific probes marked with FAMb and HEX. LOD down to 0.5%.

Starting material The kit allows the analysis of DNA extracted from fresh, frozen, formalin-fixed parain-embedded (FFPE) tissues and plasma*.

*Please note that extraction from plasma is sold separately (cat.no. H8040)

Main features Detection of the main mutations of exons 18, 19, 20, 21 of EGFR gene using 8 oligo mixes. Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene. A specific oligo control mix enables the evaluation of the quality and the quantity of the DNA in each sample.

Controls  The kit is provided with the following controls:

  • Positive control sample containing a mixture of synthetic DNA sequences that correspond to each mutation detected by this kit in a background of wild-type genomic DNA.
  • Negative control.

Sensitivity The kit allows the detection of low percentages of mutated allele in presence of high amounts of wild-type genomic DNA by real-time amplification with sequence-specific probes marked with FAMb and HEX. LOD down to 0.5%.

Starting material The kit allows the analysis of DNA extracted from fresh, frozen, formalin-fixed parain-embedded (FFPE) tissues and plasma*.

*Please note that extraction from plasma is sold separately (cat.no. H8040)

Main features Detection of the main mutations of exon 2 ( codons 12, 13), of exon 3 (codons 59, 61) and of exon 4 (codons 117, 146) of NRAS gene using 8 oligo mixes. Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene. A specific oligo control mix enables the evaluation of the quality and the quantity of the DNA in each sample.

Controls  The kit is provided with the following controls:

  • Positive control sample containing a mixture of synthetic DNA sequences that correspond to each mutation detected by this kit in a background of wild-type genomic DNA.
  • Negative control.

Sensitivity The kit allows the detection of low percentages of mutated allele in presence of high amounts of wild-type genomic DNA by real-time amplification with sequence-specific probes marked with FAMb and HEX. LOD down to 0.5%.

Starting material The kit allows the analysis of DNA extracted from fresh, frozen, formalin-fixed parain-embedded (FFPE) tissues and plasma*.

*Please note that extraction from plasma is sold separately (cat.no. H8040)

Main features Detection, by allelic discrimination, of the DPYD gene polymorphisms DPYD*2A (IVS14+1G>A,c.1905+1G>A,rs3918290), DPYD*13 (c.1679T>G,rs55886062), DPYD D949V (c.2846A>T, rs67376798) and DPYD IVS10 (c.1129–5923C>G, rs75017182),associated with the toxicity due to the treatment with Fluoropyrimidines, using 4 oligo mixes. Each mix allows the co-amplification of the mutant sequence (FAM) as well as the wild-type sequence (HEX).

Controls  The kit is provided with the following controls:

  • DPYD WT positive control: Positive control DNA containing a mixture of synthetic wild-type DNA sequences for the DPYD polymorphisms analyzed.
  • DPYD MT positive control: Positive control DNA containing a mixture of synthetic mutant DNA sequences for the DPYD polymorphisms analyzed.
  • Negative control.

Starting material The kit allows the analysis of genomic DNA extracted from whole blood.

Main features Detection, by allelic discrimination, of the UGT1A1 gene polymorphisms UGT1A1*1 (TA)6, UGT1A1*28 (TA)7, UGT1A1*36 (TA)5 and UGT1A1*37 (TA)8, associated with the toxicity due to the treatment with Irinotecan, using 1 oligo mix. UGT1A1 mix contains HEX labeled probes for UGT1A1*28 and UGT1A1*37 and FAM labeled probes for UGT1A1*1 and UGT1A1*36.

Controls  The kit is provided with the following controls:

  • UGT1A1 WT positive control: Positive control DNA containing synthetic wild-type UGT1A1*1/*1 DNA sequence.
  • UGT1A1 MT positive control: Positive control DNA containing a synthetic mutant UGT1A1*28/*28 DNA sequence.
  • Negative control.

Starting material The kit allows the analysis of genomic DNA extracted from whole blood.

Main features Detection of the main mutations of exon 2 (codons 12,13), of exon 3 (codons 61) of the genes KRAS, NRAS, HRAS and of the codons 600 and 601 of the gene BRAF using 8 oligo mixes. Each mix allows the co-amplification of one or more mutated alleles plus an endogenous control gene.

Controls  The kit is provided with the following controls:

  • Positive control sample containing a mixture of synthetic DNA sequences that correspond to each mutation detected by this kit in a background of wild-type genomic DNA.
  • Negative control.

Sensitivity The kit allows the detection of low percentages of mutated allele in presence of high amounts of wild-type genomic DNA by real-time amplification
with sequence-specific probes marked with FAM and HEX. LOD down to 0.5%.

Starting material The kit allows the analysis of DNA extracted from fresh, frozen, formalin-fixed parain-embedded (FFPE) tissues, plasma*, and cytological samples.

Download the EasyPGX qPCR system brochure
Category

Realtime PCR

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